NILE RED excellent quality manufacturing, exporter and supplier. Nile red (also known as Nile blue oxazone) is a lipophilic stain. It is produced by boiling a solution of Nile blue with sulfuric acid. Nile red has applications in cell biology, where it can be used as a membrane dye which can be readily visualised using an epifluorescence microscope with excitation and emission wavelengths usually shared with RFP. Nile red is used to localize and quantitate lipids, particularly neutral lipid droplets within cells. Nile red is almost nonfluorescent in water and other polar solvents but undergoes fluorescence enhancement and large absorption and emission blue shifts in nonpolar environments.

Technical Specifications:
C.A.S. No. 7385-67-3
M.F. C20H18N2O2
M.W. 318.38gm/mole


Solubility 0.1% (MeOH) Clear solution
Absorption maximum (MeOH) ?max 551-554nm
Absorptivity (A1%/1cm in MeOH, at ?max) >1400
Loss On Drying (110°C) <1%

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